The Department of Defense is seeking to purchase next-generation sequencing reagents for the U.S. Naval Medical Research Unit SOUTH, specifically for library preparation and sequencing on Illumina platforms. This opportunity is set aside for 8(a) sole source businesses, making it suitable for eligible small businesses in that category. The notice type is a Special Notice, indicating that interested vendors should track the opportunity rather than submit bids, as it may be awarded directly to a qualified vendor.
Analysis by Mindy, grounded in the SAM.gov notice.
Description
PERFORMANCE WORK STATEMENT
SCOPE
The laboratory of the Virology Department of the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) is looking to purchase reagents to prepare libraries using human samples that later will be sequenced in the MiSeq and MiniSeq platforms developed by Illumina, as part of the projects of next-generation sequencing (NGS) that are being conducted at this command.
BACKGROUND
The kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime ends for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high-quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run.
REQUIREMENTS
Location of work / Place of delivery
Shipment to NAMRU SOUTH – Callao, Peru
Period of performance / Delivery time
In accordance with deliverables schedule.
Deliverables schedule (if applicable)
Materials should be shipped upon award signature.
Hazardous material category.
None of the items are hazardous materials.
List of products
Cat. #
Item
Quantity
FC-110-3001
PhiX control v3 kit
2
MS-102-3003
MiSeq Reagent Kit v3 (600-cycle)
10
FC-131-1096
Nextera XT DNA Sample Preparation Kit
3
FC-131-2001
Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)
1
FC-420-1003
MiniSeq High Output Reagent Kit (300-cycles)
6
FC-131-2002
Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)
1
Applicable Standards
These reagents are very time-sensitive and have limited expiration dates, reason for which, vendor should inform final users of expiration dates as soon as they become available and request their approval prior to shipment.
Special Requirements
All materials require cold chain.
Source: SAM.gov, as posted. Verify the current solicitation before responding.
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